br Materials and methods br Results br Discussion

2018-10-20

Materials and methods Results Discussion Our results show the potential of H2A.Z to provide a highly specific biomarker for identifying carboxypeptidase in tissues that are undergoing two tightly linked processes, deterministic asymmetric self-renewal and non-random segregation (Rambhatla

br Introduction Foundational cell fate reprogramming studies

2018-10-20

Introduction Foundational cell fate reprogramming studies revealed ectopic expression of pluripotency-associated transcription factors (TF) OCT4, SOX2, KLF4, and c-MYC (OSKM) or OCT4, SOX2, NANOG, LIN28 (OSNL) in human fibroblasts (hFibs) cultured in pluripotent supportive conditions was sufficie

Mari n and colleagues previously reported that knockout of T

2018-10-20

Marión and colleagues previously reported that knockout of TP53 in human and mouse fibroblasts allows for efficient reprogramming at the expense of increased DNA damage, which was attributed to a decrease in apoptosis of DNA damaged faah inhibitors (Marión et al., 2009). In contrast, we found that t

br Discussion Through pBase mediated transposition stable ce

2018-10-20

Discussion Through pBase-mediated transposition, stable cell lines can be rapidly generated. This technology not only facilitates the investigation of cell biology in vitro but also permits the in vivo induction of requisite transgenes from transplanted cell populations. Therapeutic applications

The role of Mediator in general

2018-10-20

The role of Mediator in general versus specialized aspects of Pol II-based transcription has been the subject of debate. Work in yeast suggests that core components of Mediator are required for most Pol II-mediated transcription and that Mediator subunits are localized upstream of most promoters (An

br Looking forward Stem cell and replacement therapies have

2018-10-20

Looking forward Stem cell and replacement therapies have been extremely effective for blood diseases, including mitochondrial diseases with single or limited organ involvement such as mitochondrial neurogastrointestinal encephalomyopathy (Halter et al., 2011); however, the maternal inheritance pa

The fast advances in protocol and mechanism studies

2018-10-20

The fast advances in protocol and mechanism studies have made iN direct reprogramming a promising strategy to study and treat neural diseases. However, only a few human somatic cell types have been tested for iN induction. Whether the protocols for these somatic buy Octreotide acetate are generally

To explore the potential mechanism underlying this interesti

2018-10-20

To explore the potential mechanism underlying this interesting phenomenon, we examined the role of mTOR in this process as mTOR is well known for its role in controlling cell growth and metabolism, and more importantly, it is known to crosstalk with GSK3 (Inoki et al., 2006). Indeed, we were able re

We believe that the alteration of cell adhesion capacity

2018-10-20

We believe that the alteration of cell adhesion capacity may have important functional consequences, explaining the differences that we observed in the cell cycling and quiescence of hematopoietic progenitors. Supporting this notion, here we show that the downregulation of PTPN13 or β-catenin signif

We previously discovered that ATM

2018-10-20

We previously discovered that ATM, in addition to serving many roles in cell-cycle regulation and DNA damage response, also functions as a key epigenetic regulator in neurons, likely underlying the ataxic symptoms as well as neurodegeneration. ATM indirectly regulates nuclear-cytoplasmic shuttling o

br Acknowledgments The study is supported by

2018-10-20

Acknowledgments The study is supported by funds to W-Q.G. from the Chinese Ministry of Science and Technology (2012CB966800, 2013CB945600, and 2012CB967903), the National Natural Science Foundation of China (81130038 and 81372189), the Science and Technology Commission of Shanghai Municipality (P

Many non integrating reprogramming strategies have been inve

2018-10-20

Many non-integrating reprogramming strategies have been investigated over the past 8 years, such as minicircle plasmids, synthetic mRNA/miRNA, proteins, and small molecules. However, all these approaches are labor intensive, time consuming, and often inefficient. Recently, much attention has been fo

While this manuscript was in preparation Gutschner

2018-10-20

While this manuscript was in preparation, Gutschner et al. (2016) also described a version of SpCas9 fused to the N-terminal region of human Geminin. Although the authors reported a modest increase (28%–87%) in HR compared with unmodified SpCas9 in HEK293T cells, their Cas9-Geminin fusion provoked s

DENV as one of the most widespread vector borne viruses

2018-10-20

DENV, as one of the most widespread vector-borne viruses, infects millions of people each year worldwide. However, no clinically approved drugs are yet available against DENV. There is an urgent need to develop effective anti-DENV drugs for such treatment. In this study we tested IFN-α2a, chloroquin

Hello world!

2018-07-29